Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability
نویسندگان
چکیده
OBJECTIVE(S) The examination of the possibility of applying lower CPA- concentrations and obtaining the similar results to those using higher concentrations; as it is shown, the toxicity of the CPAs used in vitrification approach will diminish. MATERIALS AND METHODS Following vitrification/warming, oocytes were subjected to PZD/ICSI. SRs, FRs, and DRs were recorded. SRs and DRs of the embryos were monitored after vitrification/warming. IHC studies were done. Data were analyzed in comparison to the data of Exp. (experimental groups) applying 1.5 M CPA- concentrations (largely-used concentration). RESULTS The data of oocytes exposed to 1.25 M concentrated CPAs were in consistency with those exposed to 1.5 M and fresh oocytes in terms of SRs, FRs and DRs. Normal spindle and chromatin configuration is in consistence between the two experimental groups, but lower in comparison with control group. The lower the concentrations were, the less SRs, FRs, DRs were. Also, spindle organizations were more normal in comparison with the experimental groups as the concentrations decreased. The results of DRs for embryos which were exposed to 1.25 and 1.0 M concentrated CPAs were close to those vitrified with 1.5 M and fresh embryos but IHC observations in the three Exp. were significantly lower than those of fresh embryos. The results of 7.5 M concentrated CPAs solutions were significantly lower than those of the control group 1.5, 1.25 and 1.0 M treated. CONCLUSIONS Vitrification by cryotop technology using minimal volume approach increases both cooling and warming rates, therefore, the CPAs limited reduction to 1.25 and 1.0 M instead of using 1.5 M for oocytes and embryos cryotop-vitrification procedure, may be a slight adjustment.
منابع مشابه
Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability
متن کامل
O-41: Comparison of Vitrification Systems inHuman Oocytes and Evaluation of HSP A1Aand MnSOD Gene Expression Patterns
Background: The aim of this study was to compare two vitrification systems (Cryotop and OPS) on mouse MII oocytes and evaluate its effects on expression of heat shock protein A1A and MnSOD genes. Materials and Methods: Groups of about 15 oocytes without cumulus complexes (granulose, et. al.) were collected and exposed to equilibration and vitrification solutions before loading the oocytes into ...
متن کاملClosed vitrification of mouse oocytes using the CryoLogic vitrification method: A modification that improves developmental competence
OBJECTIVE To compare the mouse oocyte vitrification outcomes of the CryoLogic vitrification method (CVM) and the conventional open method using a Cryotop. Two CVM methods (original CVM and modified CVM) were tested. METHODS Mature oocytes obtained from female BDF-1 mice were vitrified by two-step exposure to equilibrium and vitrification solutions. Three vitrification protocols were tested on...
متن کاملComparison of The Effects of Vitrification on Gene Expression of Mature Mouse Oocytes Using Cryotop and Open Pulled Straw
Objective Oocyte cryopreservation is an essential part of assisted reproductive technology (ART) which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems (Cryotop and Open Pulled Straw, OPS) on mature oocytes gene expressions. MaterialsAndMethods The survival rate of metaphase II (MII) mouse oocytes were assessed after cryoprese...
متن کاملComparison of Different Vitrification Procedures on Developmental Competence of Mouse Germinal Vesicle Oocytes in the Presence or Absence of Cumulus Cells
Background An evaluation of the developmental competence of vitrified mouse germinal vesicle (GV) oocytes with various equilibration and vitrification times; in the presence or absence of cumulus cells and by comparison between the cryotop method and straws was performed. MaterialsAndMethods Mouse GV oocytes were considered in cumulus-denuded oocytes (CDOs) and cumulus-oocyte complexes (COCs) g...
متن کامل